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Are you tired of analyzing data from your mass spectrometer?

A professional group of metabolome analysis will help you with data analysis.

  • I don't have a mass spectrometer, but I would like to request sample pretreatment, measurement, and analysis.
    (1) Please request wide target lipidomics contract analysis service. Details here.
  • I have a triple quadrupole mass spectrometer, but I am in trouble because there is no MRM method.
    ②Consider purchasing a method package. Details here.
  • We are currently performing MRM measurements, but the number of samples and compounds is large, making analysis difficult. I want easy-to-use software!
    (3) Please consider purchasing Multi-ChromatoAnalysT, a package for MS chromatogram analysis. Details here.
  • Can it be used with a mass spectrometer that measures with Scan?
    Please try the Sleipnir demo which is currently under development. Details here.

Wide target lipidomics contract analysis service

We have started accepting orders for quantitative lipidomics analysis using the innovative separation analysis technology "Supercritical Fluid Chromatograph-Mass Spectrometer" (SFC-MS).

Bforce Co., Ltd. has formed a business alliance with CERI to support research and development in various fields such as the creation of new medicines and functional foods.

Calculate relative quantification of lipids

A relative quantification value of lipids can be calculated by a quantification method that utilizes SFC separation and an internal standard substance. In addition, since quantitative values are calculated, not only comparison between samples of simultaneous measurement but also comparison with past samples is possible, so it is useful for tracking changes in lipids over time (comparison with cohort studies and past studies). is.

high coverage
high identifiability

About 800 types of lipids such as general phospholipids and neutral lipids are quantified simultaneously with a highly sensitive triple quadrupole mass spectrometer.

Even the types of fatty acid side chains that make up lipids can be identified. Recognize structural isomers of the same molecular weight that differ in the constituent fatty acid side chains.

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Chromatogram example of phosphatidylcholine (PC)

We can expect to discover new knowledge through precise lipid analysis.

Based on the lipidomics technology*2 developed by Takeshi Baba Laboratory, Department of Metabolomics, Medical Institute of Bioregulation, Kyushu University, we will provide this service in cooperation with Kyushu University-originated venture BeForce Co., Ltd.

*2 Widely-targeted quantitative lipidomics method by supercritical fluid chromatography triple quadrupole mass spectrometry

 (J Lipid Res2018 Jul; 59(7): 1283-1293.)

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Wide target lipidomics method package
ver2 now on sale

A method package for wide-target lipidomics by SFC(LC) MS/MS.
In addition to the ready-to-use lipids that are the basic measurement target, it is a custom-made package that allows you to add new skeletal lipids at any time.
We program so that the MRM method is automatically constructed in a form suitable for the SFC(LC)-MS/MS used by the customer.

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Package for MS chromatogram analysis　
Multi-ChromatoAnalysT

Metabolome analysis requires many samples x many compounds, and it is very time-consuming because it is necessary to analyze a large number of MS chromatograms. By overlaying all MS chromatograms on one screen, you save a lot of time and effort required for accurate quantification.

Chromatographic conversion software for mass spectrometers

under development

Software for extracting chromatograms (so-called mass spectrograms) from Mass Specrtrum. Using this software enables quantification by chromatogram and identification by mass spectrum. Accurate masses other than integer MS are also supported.

Features of Supercritical Fluid Chromatography (SFC) Lipidomics

Kenji Baba's laboratory at Kyushu University is researching and developing methods using the SFC/MS/MS system. We have developed and announced a workflow from analysis method selection, measurement, and data analysis, and it has been applied to many studies such as cholesterolemia, arteriosclerosis, myocardial infarction, exosomes, and autophagy.

System features

  • Quantitative analysis by SFC/MS/MS and stable isotope dilution method

  • High separation of lipid classes by normal-phase mode of supercritical fluid chromatography (SFC)

  • Separation of lipid-separated species by MRM mode of tandem quadrupole MS

  • ​Introducing our software accelerates data analysis by 50 times

Comparison with conventional method

​It can measure lipid molecules comprehensively and quantitatively with high sensitivity, improving data analysis throughput and reducing false positives and false negatives.

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