Metabolome analysis services
Custom-made support from various considerations to analysis
Be-Force has a wide range of methods for metabolomics/lipidomics, which it has cultivated through its research activities at Kyushu University, as well as extensive analytical experience and analytical engineers. In addition, by utilizing software developed by Be-Force, we are able to provide analysis results quickly.
Scope of analysis
We can handle not only compounds that already have a proven track record, but also compounds that do not have a proven track record, by utilizing existing literature research and our own research capabilities, and we can handle everything from various investigations to analysis on a custom-made basis. Please contact us first to discuss your needs.
Main analysis targets and examples of equipment used
Comprehensive analysis of hydrophilic metabolites
- Analysis of mainly anionic polar metabolites (sugar phosphates, organic acids, nucleotides, nucleosides, etc.)
Over 300 compounds
⇒Ion chromatography mass spectrometry (IC/MS)
- Analysis of mainly cationic polar metabolites (amino acids, nucleic acid bases, amines, etc.)
Over 300 compounds
⇒ Pentafluorophenylpropyl column (PFPP) in reversed-phase mode LC/MS (PFPP-LC/MS)
- Acyl CoA, acylcarnitine, etc.
⇒ LC/MS using a metal-free column (ODS) (ODS-LC/MS)
"Dynamic Metabolome Analysis Reveals the Metabolic Fate of Medium-Chain Fatty Acids in AML12 Cells."
Journal of agricultural and food chemistry vol. 68,43 (2020): 11997-12010.
"A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer."
Nat Commun . 2020 Mar 17;11(1):1320.
"Remote solid cancers rewire hepatic nitrogen metabolism via host nicotinamide-N-methyltransferase."
Nat Commun . 2022 Jun 15;13(1):3346.
脂質の網羅的解析（超臨界流体クロマトグラフィー）
・脂溶性代謝物（DG，TG，CE，PA，PC，PE，PG，PI，PS，LPC，LPE，PC18:0p，PE18:0p，SM）
800化合物以上
⇒超臨界流体クロマトグラフィー質量分析（SFC/MS）
https://www.cerij.or.jp/service/03_omics/lipidomics_sfc-msms.html
弊社では一般社団法人化学物質評価研究機構(CERI)と協業を行っております。
“Widely-targeted quantitative lipidomics method by supercritical fluid chromatography triple quadrupole mass spectrometry.”
Journal of lipid research vol. 59,7 (2018): 1283-1293.
1つのメソッドでの網羅的解析
・極性代謝物分析（有機酸，ヌクレオチド，ヌクレオシド，アミノ酸，核酸塩基，アミン類など）
400化合物
⇒親水性作用陰イオンクロマトグラフィー質量分析（Unified-HILIC/AEX/MS）
“Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography Mass Spectrometry (Unified-HILIC/AEX/MS):
A Single-Run Method for Comprehensive and Simultaneous Analysis of Polar Metabolome.”
Analytical chemistry vol. 94,48 (2022): 16877-16886.
Features of the deliverables
We can deliver not only the analysis results but also the chromatogram data obtained through the analysis.
